84-2402-85　［取扱停止］TANK (TRAF Family Member-Associated NF-kappa-B Activator, TRAF-interacting Protein, I-TRAF, TANK, ITRAF, TRAF2) 50ul　226178

特徴

• The tumor necrosis factor (TNF) receptor superfamily is composed of several type I integral membrane glycoproteins that exhibit homology in their cystine- rich extracellular domains.
• Members of this family include TNF-RI and -RII, FAS, OX40, CD27, CD30 and CD40.
• Ligands for these receptors can be small, secreted proteins such as TNF, or type II integral membrane proteins, such as the CD40 ligand, CD40L.
• While the signal transduction mechanism of the TNF receptor superfamily is poorly understood, stimulation of cells with either TNF or soluble CD40L has been shown to induce the nuclear translocation of NFκB.
• Members of the TRAF family associate with activated TNF-R and CD40 and have been implicated in this process.
• The discovery of a novel protein, designated TANK, has shed light on the means by which TRAF activation of NFkB occurs.
• TANK is not only capable of binding to all three TRAFs, but also of synergizing with TRAF2 to activate the NFκB signaling cascade.
• TANK contains a regulatory carboxy terminal domain that maintains its inactivity in unstimulated cells.
• Upon its association with TRAF2, the inhibitory effect of this domain is overcome.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.