84-2402-75　［取扱停止］Tak1 (Mitogen-Activated Protein Kinase Kinase Kinase 7, Transforming Growth Factor-beta-Activated Kinase 1, TGF-beta-Activated Kinase 1, MAP3K7, TAK1) 50ul　226173

特徴

• Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways.
• These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1.
• Raf-1 is a protein with intrinsic kinase activity towards serine/threonine residues and that is widely expressed in many tissue types and cell lines.
• Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood.
• Two proteins putatively involved in this process are Ksr-1 and Tak1.
• Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction.
• Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known.
• Tak1 (TGFß-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFß stimulation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.