TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element.
Functioning as a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs), TFIID mediates promoter responses to various transcriptional activators and repressors.
TAF II p20, also known as TAF12, TAF15, TAF2J or TAFII20, is a 161 amino acid subunit of TFIID that localizes to the nucleus and contains one histone-fold domain.
Expressed ubiquitously, TAF II p20 interacts with other members of the TFIID complex and, via this interaction, plays a role in mediating transcriptional activation and repression.
Two isoforms of TAF II p20 exist due to alternative splicing events.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of TAF12. Species Crossreactivity: Human, mouse, rat