For gene transcription governed by RNA polymerase I, the human transcription factor SL1 (mouse TIF-IB) directs the assembly of initiation complexes at the prompter.
Like TFIID, which directs transcription by RNA polymerase II, SL1/TIF-IB contains the TATA-binding protein (TBP) and a set of TBP-associated factors (TAFs).
The three TAF I subunits, hTAF I p110, hTAF I p63 and hTAF p48 (or mouse TAF I p95, TAF I p68 and TAF I p48) are all integral components of SL1/TIF-IB.
The mutually exclusive binding of either TAF I or TAF II subunits to TBP is believed to direct the formation of promoter and RNA polymerase-specific complexes.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TAF I. Species Crossreactivity: Human, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to amino acids 280-336 of Human TAF I p48.
