The sense of taste provides animals with valuable information about the quality and nutritional value of food.
There are four widely accepted categories of taste perception: sweet, bitter, salty and sour.
A controversial fifth taste, known as umami or monosodium glutamate (MSG), has also been described.
A family of G protein-coupled receptors are involved in taste perception and include T1R, which is involved in sweet and umami taste perception, and T2R, which is involved in bitter taste perception.
The T1R family consists of three members: T1R1, T1R2 and T1R3.
These proteins form heterodimers, which alter the selectivity of the subunits.
The T1R2 and T1R3 heterodimer functions as a receptor for sweet taste, and recognizes several sweet-tasting molecules such as sucrose, saccharin, dulcin and acesulfame-K.
The T1R1 and T1R3 heterodimer recognizes L-amino acids to perceive umami taste.
Sweet taste transduction is carried out by two pathways.
First, sucrose and other sugars activate Gas via the T1Rs, which activates adenylyl cyclase to generate cAMP.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human T1R3. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa332-386 of human T1R3.
