Synaptotagmins are a large gene family of synaptic vesicle type III integral membrane proteins that function as regulators of both exocytosis and endocytosis and are involved in neurotransmitter secretion from small secretory vesicles.
Calcium binds to Synaptotagmin I which triggers neurotransmitter release at the synapse.
Synaptotagmin II is phosphorylated by WNK1 in a process that regulates calcium-dependent interactions.
Synaptotagmin III is involved in calcium-dependent exocytosis of secretory vesicles in endocrine cells and neurons.
Synaptotagmin IV is expressed in neuronal tissues, and has the highest mRNA levels in the hippocampus.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Synaptotagmin 1/2, phosphorylated T202/199. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human Synaptotagmin 1 around the phosphorylation site of Threonine 202.
