Sterile-20 (Ste20) is a serine/threonine kinase in Saccharomyces cerevisiae that is involved in relaying signals from G protein-coupled receptors to cyto-solic MAP kinase cascades.
Mammalian protein kinases that display sequence similarity to Ste20 are divided into two groups, the PAK subfamily and the GCK subfamily.
The PAK subfamily members contain a C-terminal catalytic domain and an N-terminal regulatory domain with a p21Rac/Cdc42-binding site, and these kinases can activate both p38 MAPK and JNK.
The GCK subfamily members contain a C-terminal regulatory domain and an N-terminal catalytic domain, and they have diverse roles in many pathways, including the activation of ERK, JNK, p38 MAPK, and caspase-3.
The mammalian Ste20-like kinases (MST kinases), also known as Krs proteins, are members of the GCK subfamily.
Ksr-1 (MST-2) and Ksr-2 (MST-1) are both direct substrates of caspase-3 that accelerate caspase-3 activation.
MST-3 is ubiquitously expressed in mammalian tissue and can phosphorylate exogenous substrates as well as itself.
MST-4 is highly expressed in placenta, thymus, and peripheral blood leukocytes, and it specifically activates ERK.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of STK3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human STK3.
