Cell type-specific expression of unique carbohydrate structures on cell surface glycoproteins and glycolipids provides information relevant to cell-cell interactions in developing and adult organisms.
Sialyltransferases contribute to the diversity of carbohydrate structures through their attachment of sialic acid in various terminal positions on glycolipid and on glycoprotein (N-linked and O-linked) carbohydrate groups.
The alpha2,3 sialyltransferase (ST3Gal-IV), also known as SIAT4-C and SI4C, shows elevated expression in brain tissues.
Sialyltransferase genes are dispersed throughout the human genome.
The human SIAT4C gene maps to human chromosome 11q24.2 and encodes St3Gal-IV.
Multiple ST3Gal sialyltransferases, including St3Gal-IV, contribute to selectin ligand formation.
Selectin ligands are glycan structures that participate in leukocyte trafficking and inflammation.
ST3Gal IV expression is down-regulated in human renal cell carcinoma (RCC) and may be one of the factors associated with the malignant progression of human RCC.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of ST3GAL4. Species Crossreactivity: Human, rat
