Arginine/serine-rich (RS) domain-containing proteins and their phosphorylation by specific protein kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells.
Two SR protein-specific kinases (SRPK, also designated SFRSK), SRPK1 and SRPK2, are highly specific for the phosphorylation of these RS proteins, thereby contributing to splicing regulation.
SRPK1 plays a role in the condensation of sperm chromatin.
SRPK2 has a stringent preference for SR dipeptides and contains a proline-rich sequence at its amino terminus.
Both SRPK1 and SRPK2 are highly expresed in testis.
SRPK1 is found exclusively in pancreas and SRPK2 is found exclusively in brain and lung.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SRPK1. Species Crossreactivity: Human
