Serotonin (also designated 5-hydroxytryptamine or 5-HT) is a molecule that functions as a neurotransmitter, a hormone and a mitogen, and it is predominantly expressed in the gut, platelets and central nervous system (CNS).
In the CNS, serotonin modulates several processes, including anxiety, sleep, appetite, behavior and drug abuse.
In platelets and gut, serotonin plays a major role in cardiovascular function and motility of the gastrointestinal tract, respectively.
Serotonin mediates its effects through several of G protein coupled receptors, designated 5-HT receptors or alternatively SR receptors.
SR-3 is a ligand-gated ion channel, whereas all other known serotonin receptor subtypes are G protein-coupled receptors.
The gene which encodes SR-3 maps to human chromosome 11q23.1-q23.2.
SR-4 mediates widespread effects in central and peripheral nervous systems.
The gene which encodes SR-4 maps to human chromosome 5q31-q33.
SR-7 belongs to the superfamily of G protein-coupled receptors.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SR-4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 146-198 of Human SR-4.
