FACT is a chromatin-specific elongation factor required for transcription of chromatin templates, and it specifically interacts with nucleosomes and histone H2A/H2B dimers, to promote nucleosome disassembly upon transcription.
FACT represents a complex between SPT16, a homologue of the Saccharomyces cerevisiae Spt16/Cdc68 protein, and the high-mobility group (HMG)-1-like protein structure-specific recognition protein-1 (SSRP-1).
Similar to other (HMG) domain containing proteins, which are characterized by their ability to bend target DNAs, SSRP1 and the murine ortholog T160, physically interact with serum response factors (SRF) and function as a positive co regulatory proteins involved in modulating SRF-dependent gene expression.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide
Specificity：Recognizes endogenous levels of human SPT16. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 932-977 of human SPT16. Swiss/UniProt Accession: Q9Y5B9.
