Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors.
Mammalian Sprouty homologs share a well-conserved cysteine-rich C-terminal domain with their Drosophila counterparts.
Both Sprouty 1 and 2 are anchored to membranes by palmitoylation, associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on Serine residues.
Upon stimulation, a subset is recruited to the leading edge of the plasma membrane.
Sprouty 2 can associate with c-Cbl, a down regulator of RTK signaling, and inhibits the activities of several growth factors.
Sprouty 2 also functions as a negative regulator of embryonic lung morphogenesis and growth.
The well-conserved C-terminus of Sprouty contains two domains which are necessary for Sprouty 2 co-localization with microtubules and translocation to membrane ruffles.
In addition, the C-terminus is required for the inhibition of cell migration and proliferation.
In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SPRY2. Species Crossreactivity: Human, mouse, rat
