Tissue damage, such as hepatic and renal injury, initiates hepatocyte growth factor activator (HGFAC)-mediated limited proteolytic activation of the inactive single-chain precursor form of HGF.
Initially, HGFAC is produced as a precursor protein, which is activated by limited proteolysis and is neutralized by specific inhibitors known as HGF activator inhibitors, designated HAIs.
HAIs belong to the Kunitz-type serine protease inhibitor family.
HAIs target HGF activator and are involved in the regulation of proteolytic activation of HGF in injured tissues.
Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate and small intestine, and fetal kidney and lung.
It translates into a 478 amino acid protein.
The human HAI-2 gene maps to chromosome 19q13.1 and encodes a 252 amino acid protein, also designated human placental bikunin or kop (Kunitz domain containing protein over-expressed in pancreatic cancer).
HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SPINT1. Species Crossreactivity: Human
