Spectrin is an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane.
Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers.
Spectrin alpha I and spectrin beta I are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta II (also designated fodrin beta) are present in other somatic cells.
The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.
Defects in the SPTB gene encoding spectrin beta I are the cause of spherocytosis type I (SPH1), a disorder characterized by severe hemolytic anemia.
Another disorder due to defects of SPTB is called elliptocytosis or elliptocytosis 3 (EL3).
The human SPTB gene maps to chromosome 14q22-q23.2 and encodes the 2,137 amino acid, 246kDa erythroid form of beta-spectrin.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Spectrin beta. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 650-700 of Human Spectrin β II.
