84-2399-15　［取扱停止］SPAM1 (Hyaluronidase PH-20, Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm Adhesion Molecule 1, Sperm Surface Protein PH-20, SPAM1, HYAL3, PH20) 100ul　225990

特徴

• Hyaluronidases (HAases or HYALs) are a family of lysosomal enzymes that are crucial for the spread of bacterial infections and of toxins present in a variety of venoms.
• HYALs may also be involved in the progression of cancer.
• In humans, six members of the hyaluronidase family have been identified.
• These proteins are significant in the degradation of hyaluronic acid (HA), which is present in body fluids, tissues and the extracellular matrix of vertebrate tissues.
• HA keeps tissues hydrated, maintains osmotic balance and promotes cell proliferation, differentiation and metastasis.
• HA is also an important structural component of cartilage and other tissues and acts as a lubricant in joints.
• SPAM1 (sperm adhesion molecule 1), also designated Hyal-PH20 or sperm surface protein PH-20, was formerly referred to as HYAL1 (HYA1).
• The current nomenclature references the functional hyaluronidase activity of SPAM1 to permit acrosome-intact sperm to penetrate through the HA-rich cumulus cell layer surrounding the oocyte.
• SPAM1 is also implicated in intracellular signaling and zona pellucida binding.
• SPAM1 is found in the epididymis and in testis, more specifically in plasma and acrosomal membranes of sperm.
• SPAM1 is a structurally unique hyaluronidase in that it is a GPI-anchored protein.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.