The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1).
Sp transcription factors share similar structures but do not share similar functions.
All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus.
Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes.
Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not.
Sp2 only weakly binds to GT boxes.
Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues.
Sp1 and Sp3,but not Sp2 or Sp4, interact with E2, a regulatory element for the beta4 subunit of neuronal nicotinic acetylcholine receptors.
Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Sp3/4. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 630-680 of Human Sp3.
