84-2399-06　［取扱停止］Sp2 (Transcription Factor Sp2, KIAA0048, SP2) 50ul　225986

特徴

• The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1).
• Sp transcription factors share similar structures but do not share similar functions.
• All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus.
• Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes.
• Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not.
• Sp2 only weakly binds to GT boxes.
• Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues.
• Sp1 and Sp3, but not Sp2 or Sp4, interact with E2, a regulatory element for the b4 subunit of neuronal nicotinic acetylcholine receptors.
• Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription.
• The gene3 encoding human Sp2 maps to chromosome 17p32.3.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.