The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities.
Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules.
Ras-GRF p140 promotes nucleotide exchange on Ras p21s but not on other members of the Ras gene superfamily.
In addition, three mammalian homologs of the Drosophila Ras-GRF, son of sevenless (Sos), have been described.
These include two from mouse, mSos 1 and mSos 2, and one from human, hSos.
Vav p95 has been reported to function as a GRF in activation of Ras by the T cell receptor and has been reported to have a domain similar to that of Dbl p115, which is a GRF specific for Cdc42Hs.
Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human SOS2. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa671-716 of human SOS2. UniProt Accession: Q07890.
