The base excision repair (BER) pathway removes incorrect bases (uracil) or damaged bases (3-methyladenine) from chromatin.
Each BER enzyme system addresses a specific type of base damage.
Uracil-DNA glycosylases, UNG2 and SMUG1 (single-strand selective monofunctional uracil DNA glycosylase) remove uracil from both double- and single-stranded DNA in nucleosomes (chromatin core particle).
The uracil-excising enzyme family shares structural and functional conservation with minimal sequence conservation.
The human SMUG1 gene maps to chromosome 12q13.13.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SMUG1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 137-182 of Human SMUG1.
