84-2398-10　［取扱停止］SMNDC1 (30 a Splicing Factor SMNrp, MGC106917, MGC112663, SMN Related Protein, SMN-Related Protein, smndc1, SMNR, SPF30, SPF30, Splicing Factor 30, survival of motor neuron-Related, Survival motor neuron Domain Containing 1, Survival motor neuron Domain Containing Protein 1, Survival motor neuron Domain-Containing Protein 1, Survival of motor neuron Related Splici ...　225935

特徴

• SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family.
• It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles.
• SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs.
• The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex).
• This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome.
• In addition, the overexpression of SPF30 can lead to apoptosis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.