The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis.
The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) along with SMC3 (also designated HCAP for human chromosome-associated protein or Bama-can), and SMC2 (also designated hCAP-E) along with SMC4 (also designated hCAP-C).
The SMC1/SMC3 complex is required for metaphase progression in mitotic cells and functions independently of the SMC2/SMC4 complex during the cell cycle.
SMC1 is ubiqitiously expressed in various human tissues, including thymus, testis and colon.
SMC3 is expressed as a nuclear protein in the colon, but can also occur as a secreted proteoglycan (called Bamacan) expressed in testis and brain.
Bamacan contains several glycosylation sites and is thought to play a role in basement membrane physiology.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SMC 1, phosphorylated S957. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from human SMC 1 around the phosphorylation site of Serine 957.
