Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling.
Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation.
Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members.
Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Smad1/5/9. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminal of Human Smad1.
Shelf Life：1year
Swiss Prot Number：Q15797/Q99717/O15198
EU Commodity Code：30021010
商品名：Smad1/5/9 (Mothers Against Decapentaplegic Homolog 1, MAD Homolog 1, Mothers Against DPP Homolog 1, JV4-1, Mad-Related Protein 1, SMAD Family Member 1, SMAD 1, Smad1, hSMAD1, Transforming Growth Factor-beta-Signaling Protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1, Mothers Against Decapentaplegic Homolog 5, MAD Homolog 5, Mothers Against DPP Homolog 5, V5-1, SMAD Family Member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5, Mothers Against Decapentaplegic Homolog 9, MAD Homolog 9, Mothers Against DPP Homolog 9, Madh6, SMAD Family Member 9, SMAD 9, Smad9, SMAD9, MADH6, MADH9) 100ul
