84-2397-13　［取扱停止］SLU7 (Pre-mRNA-Splicing Factor SLU7, hSlu7) 100ul　225883

特徴

• In order to produce correctly spliced messenger RNA, two catalytic splicing steps are required.
• After catalytic step I, a major remodeling of the spliceosome occurs to establish the active site for step II.
• During the second step of mRNA splicing, exon 1 attacks an adenine-guanine (AG) dinucleotide at the 3' splice site.
• SLU7, the human homolog of the yeast step II splice factor Slu7, is required for selection of the correct AG.
• Human SLU7 associates with the spliceosome late in the splicing pathway prior to recognition of the 3' splice site for step II.
• SLU7 depletion in HeLa nuclear extract reveals that SLU7 is required to hold exon 1 tightly within the spliceosome for attack on a prespecified AG.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.