SLPI, secretory leukocyte protease inhibitor, is an enzyme that belongs to the alpha-1 antitrypsin antiprotein family.
Produced by epithelial cells, SLPI resides in parotid secretions, bronchial, nasal and cervical mucus, and seminal fluid.
SLPI plays a primary role in the regulation of neutrophil-mediated inflammation.
It does so through proteolysis and subsequent inhibition of the leukocyte serine proteases, including the neutrophil and pancreatic proteases.
The promoter region of SLPI has been identified as binding site for IRF-1, interferon regulatory factor.
Expression of SLPI is inhibited by IRF-1 co-expression, identifying SLPI as a target of IRF-1 regulation.
SLPI also functions as a macrophage derived inhibitor of macrophage response to LPS by inhibiting the production of nitric oxide, which suggests the role of SLPI in LPS tolerance.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SLPI. Species Crossreactivity: Human
