84-2397-07　［取扱停止］SLP-76 (SH2 Domain-Containing leukocyte Protein of 76kD (SLP-76) is a hematopoietic Adapter Protein that is important in multiple biochemical Signaling pathways and necessary for T Cell Development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT a) 100ul　225880

特徴

• Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production.
• When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response.
• Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1.
• The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.