Na+/H+ exchangers-1–6 (Na+/H+ antiporters, NHE-1–6) are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume.
NHEs mediate the secondary active extrusion of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+).
Excluding NHE-1, which is ubiquitously expressed, NHE isoforms 2–6 have distinct tissue- and cell type-dependent expression and inhibitiory characteristics by amiloride analogs.
Human NHE-5 transcript (solute carrier family 9, isoform-5, SLC9A5) is an 896 amino acid protein that is expressed in brain, testis, spleen and skeletal muscle.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SLC9A5. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human SLC9A5.
