Calcium signaling in mitochondria is important in order for it to function in response to a variety of extracellular stimuli.
Signaling begins with Ca(2+) entry in mitochondria via the Ca(2+) uniporter followed by Ca(2+) activation of three dehydrogenases in the mitochondrial matrix.
ARALAR, the neuronal Ca(2+)-binding mitochondrial aspartate-glutamate carrier, has Ca(2+) binding domains facing the extramitochondrial space and functions in the malate-aspartate NADH shuttle (MAS).
ARALAR is encoded by the SLC25A12 gene and is expressed in brain and skeletal muscle.
ARALAR is required for the synthesis of brain aspartate and N-acetylaspartatemay and plays a role in myelin formation.
It is also essential for the transmission of small Ca(2+) signals to mitochondria via an increase in mitochondrial NADH.
In addition, ARALAR is implicated in conferring susceptibility to schizophrenia.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SLC25A12. Species Crossreactivity: Human, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to amino acids 435-480 of Human SLC25A12.
