Na-K-Cl cotransporters (NKCC) are channel proteins that aid in the transcellular movement of chloride across both secretory and absorptive epithelia.
NKCC1 is expressed in muscle cells, neurons, and red blood cells.
In the basolateral membrane of secretory epithelia, NKCC1 mediates active chloride secretion.
The gene encoding human NKCC1 maps to chromosome 5q23.3.
In mice, disruption of the NKCC1 gene leads to deafness and impaired balance.
NKCC2 is specifically expressed in the kidney where it mediates active reabsorption of sodium chloride in the thick ascending limb of the loop of Henle.
NKCC2 is sensitive to the clinically important diuretics furosemide and bumetanide.
The gene encoding human NKCC2 maps to chromosome 15q15-q21 and mutations in this gene lead to Bartter’s syndrome, an inherited hypokalaemic alkalosis.
NCCT is a thiazide-sensitive Na-Cl cotransporter that is primarily expressed in the distal convoluted tubule of the kidney where it accounts for a significant fraction of net renal sodium reabsorption.
The gene for human NCCT map to chromosome 16q13.
Mutations in the gene encoding NCCT cause Gitelman’s syndrome, a subset of Bartter’s syndrome.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SLC12A1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human SLC12A1.
Shelf Life：1year
Swiss Prot Number：Q13621
EU Commodity Code：30021010
商品名：SLC12A1 (BSC1, Bumetanide Sensitive sodium 3, Bumetanide-Sensitive sodium-(Potassium)-chloride Cotransporter 2, Kidney specific Na K Cl symporter, Kidney-specific Na-K-Cl symporter, MGC48843, Na K 2Cl Cotransporter, NKCC2, Potassiumchloride Cotransporter 2, S12A1_HUMAN, Slc12a1, sodium Potassium chloride Cotransporter 2, solute Carrier Family 12 (sodium/Potassium/chloride Transporters), Solute Carrier Family 12 Member 1) 100ul
