A cytoplasmic domain of ~80 amino acids was identified in the apoptosis-mediating receptors TNFR1 and Fas.
This region was determined to be necessary for the transduction of the apoptotic signal and was designated the “death domain”.
Other death domain-containing, but otherwise structurally unrelated, proteins have been identified on the basis of their ability to associate with the cytoplasmic domains of TNFR1 or FAS.
FADD (also designated MORT1) and TRADD bind to Fas and TNFR1, respectively.
RIP is a death domain-containing serine/threonine kinase that binds to TRADD.
RAIDD (also designated CRADD) was identified as a RIP binding protein.
Both RAIDD and FADD can associate with members of the caspase family, providing a link between the activation of theTNFRs and the triggering of the cysteine protease cascade.
The death domain-containing protein SIVA binds to the TNFR family member CD27 and appears to play a role in CD27 mediated apoptosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SIVA1. Species Crossreactivity: Human
