84-2395-80　［取扱停止］SIRT3 (hSIRT 3, hSIRT3, Mitochondrial Nicotinamide Adenine dinucleotide Dependent Deacetylase, NAD Dependent Deacetylase sirtuin 3 Mitochondrial, NAD-Dependent Protein Deacetylase sirtuin-3, Mitochondrial, Regulatory Protein SIR2 Homolog 3, Silent mating Type information regulation 2 S.cerevisiae Homolog 3, Sir 2 like 3, SIR 2 like Protein 3, SIR 3, SIR2 L3, Sir2 ...　225818

特徴

• The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging.
• In S.
• cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci.
• Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain.
• The human homologues, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV.
• SIRT3 is a NAD-dependent deacetylase that contains one deacetylase sirtuin-type domain.
• The SIRT3 protein is widely expressed and localizes to the mitochondira where it is processed by mitochondrial processing peptidase (MPP) to yield a final product.
• This processing is most-likely necessary for its enzymatic activity.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.