84-2395-21　［取扱停止］Shc (SHC-transforming Protein 1, SHC-transforming Protein 3, SHC-transforming Protein A, Src Homology 2 Domain-Containing-transforming Protein C1, SH2 Domain Protein C1, SHC1, SHC, SHCA) 100ul　225788

特徴

• Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity.
• These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components.
• Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate.
• Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1.
• A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1.
• An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.