The family of SR factors all contain one or more RNA recognition motifs (RRM) and an SR-rich domain.
They are not only essential for constitutive splicing, but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites.
Splicing factor arginine/serine-rich 15 (SFRS15), also designated CTD-binding SR-like protein RA4, contains one RRM and one SR-rich domains.
SFRS15 interacts with C-terminal repetitive domain (CTD) of Pol II and is believed to functionally and physically link transcription and pre-mRNA processing.
Localized to the nucleus, SFRS15 is expressed as two isoforms produced by alternative splicing.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SFRS15. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 611-660 of Human SFRS15.
