84-2394-79　［取扱停止］SFRP4 (Secreted frizzled-Related Protein 4, sFRP-4, Frizzled Protein, human endometrium, FrpHE, SFRP4, FRPHE) 50ul　225768

特徴

• The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity.
• The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins, FRP-1 (also designated SARP2), FRP-2 (also designated SARP1), FRP-3, FRP-4 and SARP3 (also designated FRP-5), have been identified.
• The Frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt.
• The Frizzled-1 gene maps to human chromosome 7q21 and is expressed in adult heart, placenta, lung, kidney, pancreas, prostate and ovary and in fetal lung and kidney.
• Frizzled-2 is expressed in adult heart and fetal brain, lung and kidney.
• The frizzled related proteins FRP-1, FRP-2, FRP-3, FRP-4 and SARP3 are secreted proteins that contain regions of homology to the cysteine-rich ligand-binding domain of frizzled and a conserved hydrophilic carboxy terminal.
• The gene encoding human SARP3 maps to chromosome 4q31.3 and is expressed in retinal pigment epithelium (RPE) and pancreas, while expression of FRP-1,2 and 4 is high in developing tissues.
• The FRPs/SARPs are involved in the Wnt signaling pathway by regulating the intracellular levels of b-catenin.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.