84-2394-67　［取扱停止］SETDB1 (ERG-Associated Protein with SET Domain, ESET, H3 K9 HMTase 4, H3 lysine 9 specific 4, H3-K9-HMTase 4, H3-K9-HMTase4, Histone H3 K9 Methyltransferase 4, Histone H3-K9 Methyltransferase 4, Histone lysine N Methyltransferase, Histone-lysine N-Methyltransferase SETDB1, KG1T, KMT1E, Lysine N-Methyltransferase 1E, MGC90670, mKIAA0067, SET Domain bifurcated 1, Se ...　225762

特徴

• ERG-associated protein with SET domain (ESET), also designated Histone H3-K9 methyltransferase 4 or SET domain bifurcated 1, is a nuclear protein belonging to the histone-lysine methyltransferase family and to the Suvar3-9 subfamily.
• It is a highly conserved protein of 150 amino acids that has been implicated in chromatin structure modulation.
• ESET is excluded from cell nucleoli and areas of condensed chromatin and can associate with the nonpericentromeric regions of chromatin.
• The gene encoding for this protein, SETDB1, maps to chromosome 1q21.
• ESET is a histone methyltransferase, methylating Lys-9 of histone H3 and mutations within the SETDB1 gene abolishes its methyltransferase activity.
• This methylation acts as a tag for epigenetic transcriptional repression by rounding up HP1 proteins to methylated histones.
• ESET is widely expressed with highest levels found in testis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.