84-2394-62　［取扱停止］SET (2PP2A, HLA DR Associated Protein II, HLA-DR-Associated Protein II, I 2PP2A, I-2PP2A, I2PP2A, IGAAD, Inhibitor of granzyme A Activated DNase, Inhibitor of granzyme A-Activated DNase, Inhibitor of GzmA-Activated DNase, Inhibitor-2 of Protein Phosphatase-2A, IPP2A2, PHAPII, Phosphatase 2A Inhibitor I2PP2A, Protein Phosphatase Type 2A Inhibitor, Protein SET, Set, ...　225759

特徴

• Protein phosphatase 2A PP2A is a major mammalian protein serine/threonine phosphatase that regulates diverse cellular processes.
• Inhibitor 1 of PP2A (I1PP2A) and inhibitor 2 of PP2A (I2PP2A), which share large sequence similarity, are heat-stable protein inhibitors of the cellular phosphatase activity of PP2A.
• I1PP2A and I2PP2A were initially characterized as putative HLA class II associated proteins Phap I and Phap II.
• These inhibitor proteins act noncompetitively to selectively inhibit PP2A, but do not affect the phosphatase activity of the related proteins PP1, PP2B and PP2C.
• The I1PP2A protein is localized to both the cytoplasm and the nucleus.
• In contrast, I2PP2A is located predominantly in the nucleus and is highly expressed in Wilms’ tumor cells.
• Transient expression of I2PP2A in HEK-293 cells leads to an increase in the DNA binding activity of the proto-oncogene c-Jun.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.