Septin 6, also known as SEPT6, is a 434 amino acid protein that belongs to the septin family.
The highly conserved septin family of GTP-binding cytoskeletal proteins is implicated in membrane transport, apoptosis, cell polarity, cell cycle regulation, cytokinesis and other cellular functions.
Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules.
Filaments are assembled from asymmetrical heterotrimers, composed of Septin 2, Septin 6 and Septin 7 that associate head-to-head to form a hexameric unit, however interaction with Septin 12 alters filament structure.
Septin 6 shares 40% identity with CDC10 and contains an ATP/GTP-binding site motif.
The Septin 6 protein demonstrates nearly ubiquitous expression, with highest level in thymus.
The Septin 6 protein is phosphorylated upon DNA damage, probably by Atm or ATR.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Septin 6. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 267-312 of Human Septin 6.
