SEPHS1 (selenophosphate synthetase 1), also known as SELD, SPS or SPS1, is a 392 amino acid member of the selenophosphate synthetase 1 family and is one of two mammalian homologs of the eubacteria selenophosphate synthetase protein SelD.
SelD is an enzyme that generates the selenium donor for the biosynthesis of selenocysteine, an amino acid that is co-translationally incorporated into selenoproteins at in-frame UGA codons.
SEPHS1 has a similar function to SelD and specifically catalyzes the formation of selenophosphate (the active selenium donor) from selenide, ATP and H2O.
Proper SEPHS1 function depends on a selenium salvage system that recycles L-selenocysteine, thereby providing the substrates for selenophosphate synthesis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of SEPHS1. Species Crossreactivity: Human, mouse, rat