84-2394-06　［取扱停止］Separase (Separin, Caspase-like Protein ESPL1, Extra spindle poles-like 1 Protein, Separase, ESPL1, ESP1, KIAA0165) 100ul　225731

特徴

• Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis.
• Once activated, separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion.
• Separase and cohesin are highly conserved from yeasts to humans.
• When the cell is not dividing, separase is prevented from cleaving cohesin through its association with another protein, securin.
• When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active separase.
• Separase is transiently activated between the two meioses and may also be involved in homolog separation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.