Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans.
Members of this family of proteins are ~750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “Semaphorin” domain of ~500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats.
The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain.
Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue.
Semaphorin 4A (SEMA4A), also designated Semaphorin B, is a type I membrane protein.
The SEMA4A gene encoding the protein localizes to chromosome 1q22.
SEMA4A provides signals to specify territories inaccessible for growing neurons, inhibiting axonal extension.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SEMA4A. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 500-546 of Human SEMA4A.
