SEMA3C (also designated Semaphorin 3C, Semaphorin E, SEMAE, SemE,sema domain, immunoglobulin domain (Ig) and short basic domain, secreted)is an ubiquitous protein of ~70kDa that mediates axonal guidance, repulsive gradients, induction of growth cone collapse and cell survival/death.
Secreted SEMA3C favors survival and neuritogenesis of cultured cerebellar granule neurons (CGNs).
SEMA3C from macrophages and fibroblasts that selectively directs against sympathetic nerve fibers may be one element responsible for reduced sympathetic innervation in rheumatoid arthritis tissue.SEMA3C mutant mice die within hours after birth from congenital cardiovascular defects consisting of interruption of the aortic arch and improper septation of the cardiac outflow tract.
SEMA3C is expressed in the cardiac outflow ract as neural crest cells and promotes crest cell migration into the proximal cardiac outflow tract.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SEMA3C. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 713-768 of Human SEMA3C.
