The Sec61 complex forms the core element of the protein translation complex in the rough endoplasmic reticulum membrane.
The complex also associates with two ubiquitous ER membrane proteins: Sec62 (also designated human translocation protein 1 or HTP1) and Sec63.
The complex forms a two-way channel that transports proteins both into the ER and back to the cytosol for degradation.
Specifically, it appears the b subunit facilitates the escort of proteins back to the cytoplasm for degradation by the proteasome or by other proteolytic systems.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SEC61B. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human SEC61B.
