The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily that is located on the apical surface of cells.
ENaC mediates sodium reabsorption in kidney, distal colon, lung, ducts of exocrine glands and other organs.
ENaC is formed by heteromultimerization of four homologous subunits, alpha, beta, y and delta.
The most frequently formed heterotetramer consists of two alpha, one beta, and one y subunit, but the alpha subunit can be replaced by a ∂ subunit.
The alphaENaC gene maps to human chromosome 12p13, and expresses a glycosylated protein.
Both the beta and yENaC genes map to human chromosome 16p12, and the yENaC transcript is detected as a glycosylated protein.
The carboxy-terminus of all ENaC subunits contains Py motifs, which interact with the ubiquitin protein ligase, Nedd4, to regulate intracellular sodium concentrations.
Gain-of-function mutations involving the Py motif cause Liddle’s syndrome, an autosomal dominant form of hypertension, resulting from excessive renal sodium absorption.
Conversely, ENaC loss-of-function mutations result in pseudohypoaldosteronism type I, a disorder characterized by salt wasting and hypotension.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of SCNN1G. Species Crossreactivity: Human, mouse, rat
