84-2393-35　［取扱停止］SCK (SHC-transforming Protein 2, Protein Sck, SHC-transforming Protein B, Src Homology 2 Domain-Containing-transforming Protein C2, SH2 Domain Protein C2, SHC2, SHCB) 100ul　225695

特徴

• Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that temporally participate in signal transduction events.
• Shc-like protein (Sck) is a neuronal adaptor protein that contains an N-terminal PTB (phosphotyrosine binding) domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain.
• Human Sck transcripts are present at high levels in liver, pancreas, prostate and ovary.
• In vascular endothelial cells, Sck participates in VEGF-induced signal transduction.
• Treatment of human umbilical vein endothelial (HUVEC) cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation.
• Applications:Suitable for use in Western Blot, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.