Secretory carrier membrane proteins (SCAMPs) are components of the post Golgi membranes and are invovled in endocytosis, vesicle recycling and membrane trafficking.
The structural features of SCAMPs include multiple N-terminal NPF repeats and four highly conserved transmembrane regions.
These NPF repeats frequently interact with EH domain proteins and aid in the budding of transport vesicles from the plasma membrane or the Golgi complex.
Endocytic budding at the plasma membrane and vesicle budding at the trans-Golgi complex facilitates binding of SCAMP proteins to EH domain proteins.
SCAMPs exist as distinct but related proteins that include SCAMP1, SCAMP2, and SCAMP3.
Tyrosine-phosphorylation by the epidermal growth factor-receptor of SCAMP1 and SCAMP3 suggests that SCAMPs are regulated by phosphorylation.
Although SCAMPs are ubiquitously expressed throughout all tissue, in neural tissue the synaptic vesicles express a particularly high concentration of SCAMP1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SCAMP1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 293-338 of Human SCAMP1.
