The human UBiquitin AcAbs to Ubiquitin Ligasestivating domain 2 (UBA2) gene is located on chromosome 19q12.
Human UBA2 could form a beta mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8 in the presence of AOS1.
The recombinant proteins, Sua1p and hUba2, form a complex.
In this complex, hUba2 binds SUMO-1, which gives the complex the activity of the SUMO-1 activating enzyme.
Therefore, UBA2 has been found to be a component of the sentrin-activating enzyme complex.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of SAE2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 598-640 of Human SAE2.
