ETO and ETO-2, which are alternatively designated MTG8 and MTG16, respectively, are members of the ETO transcription factor family .
These transcription factors are characterized by a zinc-finger domain and four conserved domains, of which domain II is required for dimerization between family members .
ETO and ETO-2 may function to mediate interactions between DNA binding proteins and transcriptional regulators, such as N-CoR.
Frequently, the t(8;21) translocation of ETO produces the AML-1/ETO oncoprotein, which consists of the first 177 amino acids of AML-1 and all but the first 30 amino acids of ETO.
AML-1/ETO expression is observed in 12–15% of acute myelogenous, M2 subtype leukemias .
The AML-1/ETO fusion proteins associate with multimeric N-CoR/mSin3/HDAC1 complexes, block differentiation and induce transcriptional repression by altering chromatin remodeling .
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of RUNX1T1. Species Crossreactivity: Human, mouse, rat
