RUNX2 (Runt-related transcription Factor) is a transcription factor that binds to its canonical consensus sequence 5’- PYGPYGGT-3’ in a number of promoters and enhancers.
They include LCK, T-cell receptor enhancers, and various bone related genes such as osteocalcin, osteopontin, and bone sialoprotein.
As a result of this, RUNX2 is involved in osteoblastic differentiation and skeletal morphogenesis.
RUNX2 contains a proline/serine/ threonine-rich region at its C-terminus that has shown to be critical for its transcriptional activity.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of RUNX-2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 246-297 of Human RUNX-2.
Shelf Life：1year
Swiss Prot Number：Q13950
EU Commodity Code：30021010
商品名：RUNX-2 (Runt-Related Transcription Factor 2, Acute Myeloid Leukemia 3 Protein, Core-Binding Factor Subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific Transcription Factor 2, OSF-2, Polyomavirus Enhancer-Binding Protein 2 alpha A Subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 Enhancer Factor 1 alpha A Subunit, SL3/AKV Core-Binding Factor alpha A Subunit, RUNX2, AML3, CBFA1, OSF2, PEBP2A) 100ul
