Ribosomal subunits are synthesized in the nucleus, and mature 40S and 60S subunits are exported stoichiometrically into the cytoplasm.
Both the 40S and 60S subunits are composed of four RNA species and ~80 structurally distinct proteins.
Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit.
Ribosomal proteins have the ability to pass through the nuclear envelope in the native state, making them the largest of the structures accomodated by the nuclear pore complexes.
The nuclear export of ribosomal subunits is a unidirectional, saturable and energy-dependent process.
Ribosomal Protein S3A, a component of the 40S subunit that belongs to the S3AE family of ribosomal proteins, localizes to the cytoplasm.RPS3A, the gene encoding for the Ribosomal Protein S3A, contains six exons and five introns spanning 5013 bp and maps to chromosome 4q31.2-q31.3.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of RPS3A. Species Crossreactivity: Human, mouse, rat