84-2391-16　［取扱停止］RPA1 (Replication Protein A 70kD DNA-Binding Subunit, RP-A p70, Replication Factor A Protein 1, Short name=RF-A Protein 1, Single-stranded DNA-Binding Protein, Cleaved into the following Chain:Replication Protein A 70kD DNA-Binding Subunit, N-Terminally processed, RPA1, REPA1, RPA70) 50ul　225585

特徴

• The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses.
• Replication protein A (RPA), a highly conserved eukaryotic protein, is a hetero-trimeric SSB.
• RPA plays an important role in DNA replication, recombination and repair.
• The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of an ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA.
• RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair.
• It can also play a role in telomere maintenance.
• The RPA 70kDa subunit binds to ssDNA and mediates interactions with many cellular and viral proteins.
• The DNA binding domain lies in the middle of RPA 70kDa subunit and comprises two structurally homologous subdomains oriented in tandem.
• RPA contains a conserved four cysteine-type zinc-finger motif, which mediates the transition of RPA-ssDNA interaction to a stable RPA-ssDNA complex in a redox-dependent manner.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.