The nuclear orphan receptors RORalpha and RORγ are members of the nuclear hormone receptor superfamily.
This family acts by directly associating with DNA sequences known as hormone response elements (HREs) and typically binds DNA as either homo- or heterodimers.
RORalpha and RORγ are unique in that they bind DNA as monomers.
RORalpha has multiple isoforms that share common DNA- and putative ligand-binding domains, but differ in their aminoterminal domains, which are generated by alternative RNA processing.
RORγ comprises a 560 amino acid protein that shares 50% amino acid identity with RORalpha and is most highly expressed in skeletal muscle.
Although these proteins are considered “orphan receptors,” due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of RORγ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 114-159 of Human RORγ.
